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biotinylated goat anti rabbit polyclonal secondary antibody  (Jackson Immuno)


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    Jackson Immuno biotinylated goat anti rabbit polyclonal secondary antibody
    Biotinylated Goat Anti Rabbit Polyclonal Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+goat+anti+rabbit+polyclonal+secondary+antibody/Biotin-SP+(long+spacer)+AffiniPure+F(ab')%E2%82%82+Fragment+Goat+Anti-Rabbit+IgG%2C+Fc+fragment+specific/pmc12571900__41590_2025_2299_MOESM1_ESM-119-18-25
    Average 93 stars, based on 34 article reviews
    biotinylated goat anti rabbit polyclonal secondary antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Saline:

    Article Title: Ketogenic diet changes microglial morphology and the hippocampal lipidomic profile differently in stress susceptible versus resistant male mice upon repeated social defeat.
    Article Snippet: Psychological stress confers an increased risk for several diseases including psychiatric conditions.. The susceptibility to psychological stress is modulated by various factors, many of them being modifiable lifestyle choices.. The ketogenic diet (KD) has emerged as a dietary regime that offers positive outcomes on mood and health status.

    Article Title: Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions
    Article Snippet: Afterward, sections were incubated with an anti-IBA1 antibody (ionized calcium-binding adapter molecule 1, 1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical) at 4 °C overnight. .. The following day, the antibody was washed out and the sections were incubated with biotinylated goat anti-rabbit polyclonal secondary antibody (1:300 in TBS cat# 111-066-046, Jackson ImmunoResearch) in Tris-buffered saline (TBS; 50 mM) for 1.5 h, followed by avidin–biotin complex solution (1:1000 in TBS; cat# PK-6100, Vector Laboratories) for 1 h at room temperature. .. Staining was revealed in Tris buffer (TB: 0.05 M, pH 8) containing 0.05% diaminobenzidine (DAB; cat# D5905-50TAB, Merck KGaA Darmstadt) and 0.015% hydrogen peroxide.

    Article Title: Ketogenic diet alters microglial morphology and changes the hippocampal lipidomic profile distinctively in stress susceptible versus resistant male mice upon repeated social defeat
    Article Snippet: Sections were then incubated in BB (10% fetal bovine serum, 3% bovine serum albumin, 0.01% Triton-X) for 1 h. Subsequently, the sections were incubated overnight at 4 °C in BB with primary antibody cocktail ([1:1000] rabbit anti-Iba1 polyclonal primary antibody (FUJIFILM, Wako Chemical, Osaka, Japan, cat#019-19741). .. After reaching RT, the sections were washed with Tris-buffered saline (TBS, 50 mM, pH 7.4) five times for 5 min and incubated in BB containing biotinylated goat anti-rabbit polyclonal secondary antibody ([1:200] Jackson ImmunoResearch, West Grove, PA, USA, cat# 111-066-046) in TBS for 1.5 h. Sections were next incubated with avidin-biotin complex solution (Vector Laboratories, Burlingame, CA, USA, cat# PK-6100,) [1:100] in TBS; for 1 h at RT. .. The staining was revealed in 0.05% diaminobenzidine (DAB; Millipore Sigma cat# D5905-50TAB,) with 0.015% H 2 O 2 in Tris-buffer (TB, pH 8.0) for 4.5 min at RT.

    Article Title: Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions.
    Article Snippet: Afterward, sections were incubated with an anti-IBA1 antibody (ionized calcium-binding adapter molecule 1, 1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical) at 4 °C overnight. .. The following day, the antibody was washed out and the sections were incubated with biotinylated goat anti-rabbit polyclonal secondary antibody (1:300 in TBS cat# 111-066-046, Jackson ImmunoResearch) in Tris-buffered saline (TBS; 50 mM) for 1.5 h, followed by avidin–biotin complex solution (1:1000 in TBS; cat# PK-6100, Vector Laboratories) for 1 h at room temperature. .. Staining was revealed in Tris buffer (TB: 0.05 M, pH 8) containing 0.05% diaminobenzidine (DAB; cat# D5905-50TAB, Merck KGaA Darmstadt) and 0.015% hydrogen peroxide.

    Article Title: Astrocytes display ultrastructural alterations and heterogeneity in the hippocampus of aged APP-PS1 mice and human post-mortem brain samples
    Article Snippet: They were then incubated overnight in a blocking buffer solution with the primary rabbit polyclonal anti-GFAP antibody (1:5000; Abcam, Cambridge, MA, USA, Ab7260) at 4 °C. .. The following day, the sections were washed in 0.01% PBS-Triton (PBS-T) and incubated with a biotinylated goat anti-rabbit polyclonal secondary antibody (1:300; Jackson ImmunoResearch, Baltimore, USA, cat# 111-066-046) in Tris-buffered saline (TBS; 50 mM, pH 7.4) for 2 h at RT. .. Afterward, the sections were washed in PBS-T and incubated for 1 h at RT in an avidin–biotin complex solution (ABC; 1:100; Vector Laboratories, Newark, USA, cat# PK-6100) in TBS.

    Incubation:

    Article Title: Ketogenic diet changes microglial morphology and the hippocampal lipidomic profile differently in stress susceptible versus resistant male mice upon repeated social defeat.
    Article Snippet: Psychological stress confers an increased risk for several diseases including psychiatric conditions.. The susceptibility to psychological stress is modulated by various factors, many of them being modifiable lifestyle choices.. The ketogenic diet (KD) has emerged as a dietary regime that offers positive outcomes on mood and health status.

    Article Title: Transcriptional and epigenetic targets of MEF2C in human microglia contribute to cellular functions related to autism risk and age-related disease
    Article Snippet: They were then incubated overnight in blocking buffer solution with a primary rabbit anti-Iba1 antibody (1:1000; FUJIFILM Wako Chemical #019– 19741) at 4°C. .. The following day, the brain sections were washed with TBS (50 mM, pH 7.4) then incubated with a biotinylated goat anti-rabbit polyclonal secondary antibody (1:300; Jackson ImmunoResearch #111-066-046) in TBS for 2 h at 22C. .. Afterwards, the sections were washed in TBS and incubated for 1 h at 22C in an avidin-biotin complex solution (ABC; 1:100; Vector Laboratories PK-6100) in TBS.

    Article Title: Integrated stress response associated with dark microglia promotes microglial lipogenesis and contributes to neurodegeneration
    Article Snippet: They were incubated overnight in a blocking buffer solution with a rabbit anti-p-eIF2α primary antibody (1:300; Cell Signaling, 3398T) at 4°C. .. The following day, the brain sections were washed 3 times for 7 min with TBS, then incubated with a biotinylated goat anti-rabbit polyclonal secondary antibody (1:300; Jackson ImmunoResearch, 111-066-046) in TBS for 2 h at RT. .. Next, sections were washed in TBS and incubated with an avidin-biotin complex solution (ABC; 1:100; Vector Laboratories, PK-6100) in TBS for 1 h at RT.

    Article Title: Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions
    Article Snippet: Afterward, sections were incubated with an anti-IBA1 antibody (ionized calcium-binding adapter molecule 1, 1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical) at 4 °C overnight. .. The following day, the antibody was washed out and the sections were incubated with biotinylated goat anti-rabbit polyclonal secondary antibody (1:300 in TBS cat# 111-066-046, Jackson ImmunoResearch) in Tris-buffered saline (TBS; 50 mM) for 1.5 h, followed by avidin–biotin complex solution (1:1000 in TBS; cat# PK-6100, Vector Laboratories) for 1 h at room temperature. .. Staining was revealed in Tris buffer (TB: 0.05 M, pH 8) containing 0.05% diaminobenzidine (DAB; cat# D5905-50TAB, Merck KGaA Darmstadt) and 0.015% hydrogen peroxide.


    Article Title: Ketogenic diet alters microglial morphology and changes the hippocampal lipidomic profile distinctively in stress susceptible versus resistant male mice upon repeated social defeat
    Article Snippet: Sections were then incubated in BB (10% fetal bovine serum, 3% bovine serum albumin, 0.01% Triton-X) for 1 h. Subsequently, the sections were incubated overnight at 4 °C in BB with primary antibody cocktail ([1:1000] rabbit anti-Iba1 polyclonal primary antibody (FUJIFILM, Wako Chemical, Osaka, Japan, cat#019-19741). .. After reaching RT, the sections were washed with Tris-buffered saline (TBS, 50 mM, pH 7.4) five times for 5 min and incubated in BB containing biotinylated goat anti-rabbit polyclonal secondary antibody ([1:200] Jackson ImmunoResearch, West Grove, PA, USA, cat# 111-066-046) in TBS for 1.5 h. Sections were next incubated with avidin-biotin complex solution (Vector Laboratories, Burlingame, CA, USA, cat# PK-6100,) [1:100] in TBS; for 1 h at RT. .. The staining was revealed in 0.05% diaminobenzidine (DAB; Millipore Sigma cat# D5905-50TAB,) with 0.015% H 2 O 2 in Tris-buffer (TB, pH 8.0) for 4.5 min at RT.

    Article Title: Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions.
    Article Snippet: Afterward, sections were incubated with an anti-IBA1 antibody (ionized calcium-binding adapter molecule 1, 1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical) at 4 °C overnight. .. The following day, the antibody was washed out and the sections were incubated with biotinylated goat anti-rabbit polyclonal secondary antibody (1:300 in TBS cat# 111-066-046, Jackson ImmunoResearch) in Tris-buffered saline (TBS; 50 mM) for 1.5 h, followed by avidin–biotin complex solution (1:1000 in TBS; cat# PK-6100, Vector Laboratories) for 1 h at room temperature. .. Staining was revealed in Tris buffer (TB: 0.05 M, pH 8) containing 0.05% diaminobenzidine (DAB; cat# D5905-50TAB, Merck KGaA Darmstadt) and 0.015% hydrogen peroxide.

    Article Title: Astrocytes display ultrastructural alterations and heterogeneity in the hippocampus of aged APP-PS1 mice and human post-mortem brain samples
    Article Snippet: They were then incubated overnight in a blocking buffer solution with the primary rabbit polyclonal anti-GFAP antibody (1:5000; Abcam, Cambridge, MA, USA, Ab7260) at 4 °C. .. The following day, the sections were washed in 0.01% PBS-Triton (PBS-T) and incubated with a biotinylated goat anti-rabbit polyclonal secondary antibody (1:300; Jackson ImmunoResearch, Baltimore, USA, cat# 111-066-046) in Tris-buffered saline (TBS; 50 mM, pH 7.4) for 2 h at RT. .. Afterward, the sections were washed in PBS-T and incubated for 1 h at RT in an avidin–biotin complex solution (ABC; 1:100; Vector Laboratories, Newark, USA, cat# PK-6100) in TBS.

    Avidin-Biotin Assay:

    Article Title: Ketogenic diet changes microglial morphology and the hippocampal lipidomic profile differently in stress susceptible versus resistant male mice upon repeated social defeat.
    Article Snippet: Psychological stress confers an increased risk for several diseases including psychiatric conditions.. The susceptibility to psychological stress is modulated by various factors, many of them being modifiable lifestyle choices.. The ketogenic diet (KD) has emerged as a dietary regime that offers positive outcomes on mood and health status.

    Article Title: Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions
    Article Snippet: Afterward, sections were incubated with an anti-IBA1 antibody (ionized calcium-binding adapter molecule 1, 1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical) at 4 °C overnight. .. The following day, the antibody was washed out and the sections were incubated with biotinylated goat anti-rabbit polyclonal secondary antibody (1:300 in TBS cat# 111-066-046, Jackson ImmunoResearch) in Tris-buffered saline (TBS; 50 mM) for 1.5 h, followed by avidin–biotin complex solution (1:1000 in TBS; cat# PK-6100, Vector Laboratories) for 1 h at room temperature. .. Staining was revealed in Tris buffer (TB: 0.05 M, pH 8) containing 0.05% diaminobenzidine (DAB; cat# D5905-50TAB, Merck KGaA Darmstadt) and 0.015% hydrogen peroxide.

    Article Title: Ketogenic diet alters microglial morphology and changes the hippocampal lipidomic profile distinctively in stress susceptible versus resistant male mice upon repeated social defeat
    Article Snippet: Sections were then incubated in BB (10% fetal bovine serum, 3% bovine serum albumin, 0.01% Triton-X) for 1 h. Subsequently, the sections were incubated overnight at 4 °C in BB with primary antibody cocktail ([1:1000] rabbit anti-Iba1 polyclonal primary antibody (FUJIFILM, Wako Chemical, Osaka, Japan, cat#019-19741). .. After reaching RT, the sections were washed with Tris-buffered saline (TBS, 50 mM, pH 7.4) five times for 5 min and incubated in BB containing biotinylated goat anti-rabbit polyclonal secondary antibody ([1:200] Jackson ImmunoResearch, West Grove, PA, USA, cat# 111-066-046) in TBS for 1.5 h. Sections were next incubated with avidin-biotin complex solution (Vector Laboratories, Burlingame, CA, USA, cat# PK-6100,) [1:100] in TBS; for 1 h at RT. .. The staining was revealed in 0.05% diaminobenzidine (DAB; Millipore Sigma cat# D5905-50TAB,) with 0.015% H 2 O 2 in Tris-buffer (TB, pH 8.0) for 4.5 min at RT.

    Article Title: Microglial diversity along the hippocampal longitudinal axis impacts synaptic plasticity in adult male mice under homeostatic conditions.
    Article Snippet: Afterward, sections were incubated with an anti-IBA1 antibody (ionized calcium-binding adapter molecule 1, 1:1000 in blocking buffer, cat# 019-19741, FUJIFILM Wako Chemical) at 4 °C overnight. .. The following day, the antibody was washed out and the sections were incubated with biotinylated goat anti-rabbit polyclonal secondary antibody (1:300 in TBS cat# 111-066-046, Jackson ImmunoResearch) in Tris-buffered saline (TBS; 50 mM) for 1.5 h, followed by avidin–biotin complex solution (1:1000 in TBS; cat# PK-6100, Vector Laboratories) for 1 h at room temperature. .. Staining was revealed in Tris buffer (TB: 0.05 M, pH 8) containing 0.05% diaminobenzidine (DAB; cat# D5905-50TAB, Merck KGaA Darmstadt) and 0.015% hydrogen peroxide.

    Blocking Assay:




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